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KMID : 0613820100200121902
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Journal of Life Science
2010 Volume.20 No. 12 p.1902 ~ p.1905
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Production of All-Female using Sex-Reversal Gynogenetic Diploid in Rainbow Trout, Oncorhynchus mykiss
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Lee Cheul-Ho
Kim Dae-Jung
Jeong Chang-Hwa
Lee Chae-Sung
Kim Dong-Soo
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Abstract
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This study was conducted to increase the efficiency of farming practice in rainbow trout, Oncorhynchus mykiss, by sex reversal and chromosome-set manipulation techniques. Induction of sex-reversed gynogenetic diploid rainbow trout males and mass production of all-female rainbow trout by genetic sex reversal was performed. Phenotypic males in the gynogenetic diploid group were induced successfully by dietary administration of 5 ㎎ of 17 alpha-methyltestosterone per kg diet for 82 days. All females were produced by crossing between normal female and sex-reversed gynogenetic diploid male rainbow trout.
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KEYWORD
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Gynogenetic diploid, sex-reversal, all-female rainbow trout, 17 alpha-methyltestosterone
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